gatk4/gatk4_genomicsdbimport

variant calling
joint genotyping
germline
GATK
GATK4
GVCF
GenomicsDB

Description

Imports single-sample GVCFs into a GenomicsDB before joint genotyping.

GenomicsDBImport reads GVCF files produced by gatk4 HaplotypeCaller (or
similar tools) and consolidates them into a GenomicsDB datastore, a
scalable alternative to gatk4 CombineGVCFs for cohorts with large
numbers of samples. The initial import creates a new GenomicsDB workspace.

Type

bash_script

License

Apache-2.0

Keywords

variant calling
joint genotyping
germline
GATK
GATK4
GVCF
GenomicsDB

Contributors

Run this component

Run the following command to execute this component with Nextflow:

cat > params.yaml <<'EOM'  
genomicsdb_workspace_path: "$id.$key.genomicsdb_workspace_path.genomicsdb_workspace"  
id: "run"  
publish_dir: "output/"  
EOM

nextflow run https://packages.viash-hub.com/vsh/biobox.git \  
  -revision v0.5.0 \  
  -main-script target/nextflow/gatk4/gatk4_genomicsdbimport/main.nf \  
  -params-file params.yaml  

GATK Engine Options

Name
Type & Properties
--interval_padding
-ip
integer
--sites_only_vcf_output
boolean_true
--create_output_variant_index
-OVI
boolean
--create_output_bam_index
-OBI
boolean
--output_cram_version
string
--read_filter
-RF
string
multiple
--disable_read_filter
-DF
string
multiple
--disable_tool_default_read_filters
boolean_true
--disable_sequence_dictionary_validation
boolean_true

Input

Name
Type & Properties
--variant
-V
file
multiple
--sample_name_map
file
--intervals
-L
file
required

Output

Name
Type & Properties
--genomicsdb_workspace_path
file
required
output

Options

Name
Type & Properties
--avoid_nio
boolean_true
--batch_size
integer
--bypass_feature_reader
boolean_true
--consolidate
boolean_true
--genomicsdb_segment_size
integer
--genomicsdb_shared_posixfs_optimizations
boolean_true
--genomicsdb_vcf_buffer_size
integer
--header
file
--max_num_intervals_to_import_in_parallel
integer
--merge_contigs_into_num_partitions
integer
--merge_input_intervals
boolean_true
--overwrite_existing_genomicsdb_workspace
boolean_true
--reader_threads
integer
--validate_sample_name_map
boolean_true

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Viash Hub is a platform developed by Data Intuitive, a Belgian-based bioinformatics company specializing in data workflow development and deployment.