High-throughput RNA-seq

htrnaseq is a collection of workflows for the end-to-end processinging of high-throughput RNA-seq data.

Overview of Functionality

This workflow is designed to process high-throughput RNA-seq data, where every well of a microarray plate is a sample. A fasta file provided as input defines the mapping between sample barcodes and wells.

The full workflow is split in two major subworkflows that can be run independently:

  • Well-demultiplexing: Split the input (plate/pool level) fastq files per well.
  • Mapping, counting and QC: Run per-well mapping, counting and generate QC reports.

Input for the workflow has to be fastq files. For bcl or other formats, the demultiplex workflow needs to be run first.

Workflows == modules

Viash workflows are built on a modular architecture where components and workflows are fully equivalent. Each component can be executed as a stand-alone workflow, while any workflow can be seamlessly integrated as a dependency of another workflow. This design enables flexible customization and recombination to address diverse analytical needs.